Ppara Knockout cell line (Raw 264.7)

Ppara Knockout cell line (Raw 264.7) Order

Catalog#

YKO-MT0141

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Ppara Knockout cell line (Raw 264.7)

Species

Mouse

Gene Name

Ppara

Gene ID

19013

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 3 (based on transcript Ppara-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ppara gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ppara gene knockout Ppara Knockout cell line (Raw 264.7) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Ppara Knockout cell line (Raw 264.7)

Catalog#

YKO-MT0141

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Ppara Knockout cell line (Raw 264.7)

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